• Facebook
  • Facebook

Av. Indianópolis, 1960 | Planalto Paulista | Sao Paulo | SP

55 (11) 5581-2045 / 5072-4150 | (11) 99666-1455

Diretor Clínico: Dr. Raul Eid Nakano - CRM 46.514

Resultados para: first test tube baby

Ana Paula Caldeira - The first test tube baby in Brazil

The first test-tube baby in Brazil

Ana Paula Caldeira - The first test tube baby in Brazil

In 1984 came the gynecologist Dr. Milton Nakamura announce the arrival of Anna Paula Caldeira, the first test tube baby in Brazil.

After a profusion of births and ads in various countries, which signaled to the scientific community the field of technical, a In Vitro Fertilization has become a common medical procedure.

Currently in Brazil, there are about 150 human reproduction clinics, which are designed almost 4.000 babies per year. And this number is growing, thanks to technology that develops every day.

Today, women who can not ovulate more pregnant via donor eggs. Men who do not produce mature sperm may resort to a technique that allows to remove the pre-sperm cells and mature them, so that subsequently they are used in IVF.

Louise Brown First Test Tube Baby Humanity

The History of the First Test Tube Baby

Louise Brown a Primeira bebê de Proveta da Humanidade

There 35 year born, Louise Brown, the first test tube baby of humanity.

His birth, The madrugada of 25 July 1978, caused much uproar in Oldham General Hospital, near Manchester, England, and the scientific community. What previously seemed impossible just become reality.

With a blockage in the tubes, mother, Leslie Brown, only managed conceive found when the embryologist Robert Edwards and or ginecologista Patrick Steptoe. It took at least 50 that attempts to avenge the embryo that would become Louise Brown.

View all news is always afraid. On occasion, was not different. The media and the scientific community viewed with distrust done Patrick Steptoe and Robert Edwards. From então, test tube babies, donation and receipt of human reproductive material, freezing and discarding of embryos, cloning, genetic medicine, Bioethics, Biolaw, Dentre other topics, have left more than rely on broad exploration media.

Amid a flurry of critical, observers and hopeful couples, research continued to advance. In 1981, Doctor Howard Jones announced the birth of first test tube baby U.S. us: Elizabeth Jordan Carr.

Ferticlin Clinic for Human Reproduction and Fertility in Sao Paulo

FERTICLIN: Reference in Human Reproduction in Brazil

Ferticlin Clinic for Human Reproduction and Fertility in Sao Paulo

Full Clinic 25 years with thousands of success stories

With the aging of the female population in Brazil, the task of conceive tends to be increasingly difficult. This process is directly related to woman's childbearing years, that is delaying the dream of being a mother to the detriment of his career.

In this scenario, demand for specialized medical clinics and is an irreversible trend in the market. A Ferticlin, located in São Paulo ,and founded há 25 years by the doctor Dr. Raul Nakano is one of the references in this area.

After completing Graduation and Residency in Obstetrics in the Faculty of Medicine of Ribeirão Preto – USP, the young doctor Dr. Raul Nakano was studying in Japan, where specialized em Human Reproduction in two of the most prestigious universities in the world, a Kanazawa University (Kanazawa-Japan ) e a Keio University, ( Tokyo).

It was just the beginning of a brilliant career clinic that had momentum on his return to Brazil, where he was Clinical Director of the Center for Family Planning São Paulo ( CPF SP), located in the state capital, and commanded by Dr. Milton Nakamura (In Memoriam), responsible for the birth of Brazilian first test tube baby, in 1984.

D. Raul Nanako

Currently Dr. Raul Nakano is Clinical Director of FertiClin, a highly Clinic for Human Reproduction, located in Sao Paulo, established by the physician for more than 25 years. A FertiClin has all necessary infrastructure compatible with any Medical Center in Human Reproduction Europe, Japan and the United States.

On site, you can perform many complex procedures since it has the charge of their own Assisted Reproduction Laboratory , can perform procedures like: Oocyte freezing, Oocyte thawing, IVF, Artificial Insemination, Puncture epididymis, Fertilization by ICSI, Induction of Ovulation and Egg Donation, Oocyte Preservation, others.

“We are concerned to attend and bring any clarification of our treatments, Professional, structure and concepts of our work, maintaining quality and excellence in every detail. Today we are recognized and certified by the competent organ in human reproduction”, reveals Dr. Raul which annually participates in the main Congress Human Fertilisation national and international.


The 20 Articles that changed the world of assisted reproduction.

There is controversy over the first test tube baby, nor Birth first baby via artificial insemination. But, many discoveries in Assisted Reproduction still lost in the literature, its meaning, sometimes, forgotten. Hence Hans Evers lançou um artigo sobre ‘100 artigos para ler antes de de morrer ‘. caused so much interest that we separated the top 20 articles Hans Evers.

1. At the start
Well. . . more precisely, where to start? The history of FIV humana certainly began with Robert Edwards, that in summer 1965 spent six weeks with Howard and Georgeanna Jones, em Baltimore, where, Howard and according to a report although “negative” Edwards said he: “it seems somewhat doubtful that human fertilization was actually obtained at the Johns Hopkins Hospital by Bob and his associates.

Hans Evers: Excellent monograph Bob. Designing the Human Being Female showed me the way to reproductive medicine. I devoured the article often. The first time Bob remembered my name my heart beat faster.

Edwards RG, Donahue RP, Baramki TA,
Jones HW. Preliminary attempts to fertilize
human oocytes matured in vitro. Am J
Obstet Gynecol 1966; 96: 192–200.


2. Induction of ovulation
A first pregnancy after induction of ovulation with hMG (Pergonal) was in Israel 1961. O grupo Carl Gemzell reportou a first pregnancy gonadotropin in the previous year.

Hans Evers: There was PubMed(store medical articles) na decade 1960, why not have much impact. It was published in obscure journals – why raised doubts about the pioneering.

Lunenfeld B, Sulimovici S, Rabau E, Eshkol A. L indução’
ovulação in the pituitary amenorrhea. Franco C R Soc
Gynecol 1962; 32: 407-415.
Gemzell CA, Diczfalusy E, Till Inger G. Human follicle hipófise
stimulating (FSH). Ciba Found colloq Endocrinol 1960;
13: 191.


3. The first IVF pregnancy
O grupo Melbourne de Carl Wood em 1973 described two gravidez IVF, Both lost in less than a week. According to Alan Trounson, I would join the group in 1977, these pregnancies “chemical”, showed increased levels of hCG and showed that IVF embryos could develop in vivo and, probably, initiate initiate deployment. It is also likely that Edwards and Steptoe had begun embryo transfer, but did not report anything at the time and with no sign of implantation, except for some premature abortions.

Hans Evers: The findings of Assisted Reproduction have followed the same path; a first pregnancy Biochemistry, then abortion, next ectopic, and, finally, someone in a report can boast with the first birth.

De Kretzer D, P Dennis, Hudson B, et al.
Transfer of a human zygote. Lancet 1973;
302: 728-729.


4. The first birth
Louise Brown, was born just before midnight in Oldham, no UK. Weighing 2,61 kg. The baby was born by cesarean. From então, estimates are that more than 4 million IVF babies have been born worldwide.

Hans Evers: Why the Swedes have never been brave enough to give Edwards and Steptoe Nobel Prize? IVF has provided more insight into his life and produced more quality and life years than any other medical treatment.

Steptoe PC, Edwards RG. Birth after the
reimplantation of a human embryo. Lancet
1978; 2: 366.


5. Gonadotropins in IVF
Although Louise Brown and Baby Australia solids having the first IVF nasecerem following the egg retrieval following a natural cycle, the first IVF birth in the U.S. was a cycle stimulated with hMG; Trounson Melboure had described in the first few cycles stimulated with clomiphene citrate.

Lopata A, Brown J, Leeton J, et al. In vitro fertilization of
preovulatory oocytes and embryo transfer in infertile patients
treated with clomiphene and HCG. Fertil Steril 1978; 30: 27–35.

Trounson AO, Leeton JF, Wood C, et al. Pregnancies in humans
by fertilization in vitro and embryo transfer in the controlled
ovulatory cycle. Science 1981; 212:6 81–682.

Jones HW, Jones GS, Andrews MC, et al. The program for in
vitro fertilization at Norfolk. Fertil Steril 1982; 38: 14–21.


6. Aspiration GUIADA por ultrassom
Oocyte guided by ultrasound – and not from puncture laparoscopic – was first described in 1981 by Suzan Lenz and colleagues, Copenhagen. An ultrasound scanner with a transducer puncture a steel needle guided through a bladder filled with ovum. The authors later described technique as “atraumatic and cheap and, therefore, valuable for harvesting oocytes for in vitro fertilization.

Hans Evers: Real progress in IVF came naturally from monitoring vaginal ultrasound. And that was introduced by Matts Wikland Group in Gothenburg. From now on, IVF would be a less invasive procedure.

Lenz S, Lauritsen JG, Kjellow M.
Collection of human oocytes for in vitro
fertilisation by ultrasonically guided

Same N, Friberg J, Fullan N, et al.
Egg retrieval for in vitro fertilisation by
sonographically controlled vaginal
culdocentesis. Lancet 1983; 322: 508-509.

Wikland M, Nilsson L, Hamberger L.
The use of ultrasound in a human in vitro
fertilization program. Ultrasound Med Biol
1983; 2: 609-613.


7. Oocyte Donation
The first report of a pregnancy resulting from the transfer of a donor eggs fertilized Monash shaft Group in 1983, whose report the BMJ raised the question of what to do with excess eggs and embryos generated in a stimulated IVF cycle: “If more oocytes are retrieved are required, they can be left non-fertilized, preserved, or donated to a couple who oocytes receiver can not be obtained.
Hans Evers: The uterus is an organ demanding. Provide you with estrogens and progesterone and implantation vai leave a young embryo donors, even at age 65 years. Alan Trounson é um ícone. Of sheep (in 1974) he was for cows(1976), horses (1976), rabbits (1977), times (1980) and human (1980). From follicles eggs to embryos for sperm stem cells. And finally, law, ethics, religion and clinical translation in century 21.
Trounson A, Leeton J, Besanko M, et al.
Pregnancy established in an infertile patient
after transfer of a donated embryo
fertilised in vitro. Br Med J 1983; 286:

8. Cryopreservation of embryos
Gerard Sailmaker (1936 -2002), pictured with Robert Edwards, in 1986, recorded in his laboratory in Rotterdam that a book
human embryo was frozen in July 1979 and thawed October, survived a blastomere. Of frozen embryos Three patients in February 1983, a transfer in May resulted in the birth of monozygotic. Zeilmaker recorded that his birth, Netherlands not, held up considerably before that a baby born in Australia.

Hans Evers: Freezing of embryos High quality is now a key requirement for the clinical applicability of the transfer of a single embryo (Case), one technique introduced by assisted reproduction purposes that physiologised. Through ESET and cryopreservation, IVF has become finally a safe and effective way to help infertile couples.

Trounson A, Mohr L. Human pregnancy following
cryopreservation, thawing and transfer of an eight-cell embryo.
Nature 1983; 305: 707-709.

Sailmaker GH, Alberda AT, Ghent I, et al. Two pregnancies
following transfer of intact frozen-thawed embryos. Fertil Steril
1984; 42: 293-296.

Vilska S, A Tiitinen, Hyden-Granskog C, Hovatta O. Elective
transfer of one embryo results in an acceptable pregnancy rate and
eliminates the risk of multiple birth. Hum Reprod 1999; 14: 2392-


9. GnRH agonists in IVF cycles
After administration of pulsatile GnRH was created as a safe and effective way to treat hypogonadism hypogonadal anovulation, reports of its use for the prevention of an untimely LH surge during ovarian stimulation appeared in the early 80. Pituitary regulation resulted reduce cancellation fees, IVF outcome improved and more convenient time
uptake. The use of GnRH agonists in combination with gonadotropins in IVF was highlighted by the group Howard Jacobs, em London.

Hans Evers: Urine samples every four hours, detection of LH-waves in four hours, performing laparoscopic escuridão hours nas primeiras, seven days per week, Everything changed with ovulation in GnRH agonist when; run no side effects became available histaminergic. IVF have safer and more convenient.

Fleming R, Adam AH, Barlow DH, et al.
A new systematic treatment for infertile
women with abnormal hormone profiles.
Br J Obstet Gynaecol 1982; 89: 80–83.

RN Porter, Smith W, Craft IL, et al.
Induction of ovulation for in vitro
fertilisation using buserelin and
gonadotropins. Lancet 1984; 324: 1284-


10. Vitrification and cryopreservation of oocytes
The first successful attempt at freezing and thawing of a human egg was reported by Christopher Chen in Adelaide 1986. A twin pregnancy was achieved after fertilization and transfer. Chen relataram this 80% two 40 oocytes
frozen (a -196oC) showed morphological survival after thawing. Thirty were inseminated, 83%
maintained their ability to be fertilized, and 60% passed cleavage division.

Chen C. Pregnancy after human oocyte cryopreservation.
Lancet 1986; 327: 884-886.

Experiments with Vitrification of embryos animal models (times, date sheep) dating from the mid- 1980. In 1990 Gordts Stephan and colleagues in Leuven, Belgium, described “feezing Ultrarapid ‘ and defrost 237 human oocytes fertilized, which 34 were transferred into 20 patients, four of which became pregnant. The first birth following vitrification of a human egg was reported by Gianaroli and working with colleagues from Bologna in Trounson 1999.

Gordts S, Roziers P, Field R, Noto V. Survival and pregnancy
outcome after ultrarapid freezing of human embryos. Fertil Steril
1990; 53: 469-472.

Kuleshova L, The Gianaroli, Magli C, et al. Birth following
vitrification of a small number of human oocytes: case report.
Hum Reprod 1999; 14: 3077-3079.


11. GIFT
The first pregnancy after gamete intrafallopian transfer (GIFT) was notified of California 1984, with a live birth announcement from the same group the following year. Two years earlier Ian Craft in London reported a pregnancy after the transfer of gametes to the uterus.

Hans Evers: Must have been the symbol seducer who made this so successful in some parts of the world (especially in Australia). How else could you explain the appeal of a technique that still required general anesthesia and laparoscopy?

Craft I, McLeod F, Green S, et al. Human pregnancy following
oocyte and sperm transfer to the uterus. Lancet 1982; 319: 1031-

Asch RH, Ellsworth LR, JP Balmaceda, Wong PC. Pregnancy
after translaparoscopic gamete intrafallopian transfer. Lancet
1984; 324: 1034-1035.

Asch RH, Ellsworth LR, JP Balmaceda, Wong PC. Birth
following gamete intrafallopian transfer. Lancet 1985; 326: 163.


12. ZIFT
Zygote intrafallopian transfer, Another technique for transfer tube, was first reported by Paul Devroey Brussels 1986 and was said to combine the advantages of IVF (evidence of fertilization, deletion of polyploid embryonic) with the advantages of the present (higher rates of pregnancy).

Hans Evers: GIFT paved the way for all sorts of combinations and permutations: ZIFT, BAD, TET, transferring gametes, zygotes or embryos through the vagina, a bexiga, o tubo. Not all were unequivocally successful, but this should not distract from the fundamental contributions of Brussels team André Van Steirteghem and Paul Devroey to the development of our specialty.

Devroey P, Braeckmans P, Smitz J, et al.
Pregnancy after translaparoscopic zygote
intra-Fallopian transfer in a patient with
sperm antibodies. Lancet 1986; 327: 1329


13. Microinjection and male infertilidade
The first report of a pregnancy after injection of a single sperm cell beneath the zona pellucida of the oocyte group came from Monash 1987, who diz: “the technique has profound implications for the possible treatment of severe male infertility. The first birth after insemination subzonal (SUZI) came from Singapore next year. Male infertility was then
defined as the largest cause of infertile, accounting for about 30% Two cases (in accordance with Hull et al, 1986).

Laws-King A, Trounson A, Sathananthan H, Kola In.
Fertilization of human oocytes by microinjection of a single
spermatozoon under the zona pellucida. Fertil Steril 1987; 48:

SC, Bongso A, Ratnam SS, et al. Pregnancy after transfer of
sperm under zona. Lancet 1988; 332: 790.


14. Embryo transfer guided by ultrasound
The use of ultrasound to guide Embryo transfer was described by Strickler et al em 1985. Transfer ultrasound was found to be associated with easier and less distortion than the catheter 'blind ‘ transfers that have been used so far.

Hans Evers: As their world was guided by U.S. ET? Everyone did it, seemed to work, but she really offer something extra? Not in the hands of an experienced operator, apparently.

Strickler RC, Christianson C, Crane JP, et al. Ultrasound
guidance for human embryo transfer. Fertil Steril 1985; 43: 54–61

Kosmas IP, Janssens R, Munch L, et al. Ulrasound-guided
embryo transfer does not offer any benefit in clinical outcome: a
randomized controlled trial. Hum Reprod 2008; 23: 457-458.


15. PZD area and perfuração
The first description of partial dissection area (PZD) human oocytes to encourage penetration of the sperm in IVF (especially for male infertility) was first reported from Atlanta by the group of Jacques Cohen, a report on the perforation followed in the same year. Eventually, as a Cochrane Review would show, ICSI was found to be much more efficient technique Male infertilitya tha PZD, perfuração area and Suzi.

Cohen J. Malter H. Fehilly C, et al.
Implantation of embryos after partial
opening of oocyte zona pellucida to
facilitate sperm penetration. Lancet 1988;
332: 162.

Gordon JW, Grunfeld J, GJ Garrisi, and
to the. Fertilization of human oocytes by sperm
from infertile males after zona pellucida
drilling. Fertil Steril 1988; 50: 68-73.


16. ICSI
ICSI was developed accidentally slightly – at the VUB in Brussels and quickly took over as Suzi microinjection technique of choice for the design of male infertility. In 2006, only 14 years after Brussels, reported the first births, About two thirds of all fertilization in ART Europe was ICSI.

Hans Evers: A revolution! As they have the courage to do it? And on the chromosomes and axes? What we do know is that 1-2% of all children born today are the result of ICSI and they seem to do well. Does it still have sperm banks, if not for ICSI?

Palermo G, Joris H, Devroey P, Of
Steirteghem AC. Pregnancies after
intracytoplasmic injection of single
spermatozoon into an oocyte. Lancet 1992;


17. PGD
The technique of sexing embryos from the DNA of a cell biopsy by amplification of a repeating sequence specific to the Y chromosome was first reported by Alan Handyside and colleagues in London, who described the technique as “can be valuable for couples at risk of transmitting X-linked disease “. The same group reported the first pregnancy using the same technique in couples at risk of transmitting X-linked recessive diseases.

Hans Evers: PGD ​​was a huge step forward, both for our understanding of early embryonic development and clinical care infertility. His nephew marketed, PGS, was nipped in the bud by several robust clinical trials.

Handyside AH, Pattinson JK, Penketh RJ, et al. Biopsy of
human preimplantation embryos and sexing by DNA
amplification. Lancet 1988; 1: 347-349.


18. Maturation in vitro
Cha and colleagues, Seoul were the first to describe a pregnancy from an in vitro oocyte maturation. The newspaper reported that 270 Immature oocytes were incubated with a mature follicular fluid or fetal cord serum, the first having a higher maturation rate that the last. Five embryos transferred to a woman with FOP resulted in the birth of “healthy” Meninas triplete.

Hans Evers: Soon after the first few publications in IVM (ovinos e bovinos), animal models, the first attempt was human
announced. Various other ensued. The obstetric and perinatal outcomes were good,the average weight was normal, and child development has hitherto been imperceptible.

Cha KY, Koo JJ, Ko JJ, et al. Pregnancy
after in vitro fertilization of human
follicular oocytes collected from
nonstimulated cycles, their culture in vitro
and their transfer in a donor oocyte
program. Fertil Steril 1991; 55: 109-113.


19. Surgical sperm
The first report of a live biths following an aspiration technique sperm shaft group Irvine Ricardo Asch work Sherman Silber, in 1988. The same group later confirmed the genetic link between cystic fibrosis and CAVD, while Silber, working with VUB Brussels, reported the first pregnancy with sperm obtained from testicular biopsies in men with obstructive azoospermia or CAVD.

Hans Evers: Spineless, no glory – or chance favors the prepared mind? Immature sperm in fertility treatments human. Every cloud has a lining Silber.

Silver SJ, From Steirteghem AC, Liu J, and
to the. High fertilization and pregnancy rate
after intracytoplasmic sperm injection with
spermatozoa obtained from testicle biopsy.
Hum Reprod 1995; 10: 148-152.

Patrick P, Silver S, Ord T, et al. Two
births after microsurgical sperm aspiration
in congenital absence of vas deferens.
Lancet 1988; 332: 1362.


20. Fertility preservation
The restoration of fertility after transplant ovarian frozen, thawed tissue was first reported in sheep, in 1994, but not to 2000, foi autotransplante ovariano (orthotopic and heterotopic), described on a trial basis in human. According to recent reports, has thus far, nine deliveries of pregnancies derived from frozen-thawed ovarian tissue.

Hans Evers: Fertility preservation by freezing eggs or ovaries of ART is the answer to increasingly successful cancer treatments. His “social” application – in older women who have not yet found their Sr. Right – is still elusive. A 1-5% chance of an egg removed from becoming pregnant would require between one and five 25 pick-up procedures at one year of age 35-40 to care dela “safe fertility”.

Oktay K, Karlikaya G. Ovarian
function after transplantation of frozen,
banked autologous ovarian tissue. N Engl J
With 2000; 342: 1919.

Adapted by: FertlclinAssisted Fertility Center.